Cell type: B lymphoblast Disease: plasmacytoma; myeloma
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The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus. The cells are resistant to 8-azaguanine and do not produce immunoglobulin. The line can be used a fusion partner for making B cell hybridomas. Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium renewal: Every 2 to 3 days
culture medium 95%; DMSO, 5%
8 to 10 hrs
Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002 recommended serum: ATCC 30-2020
1092: de St.Groth SF , Scheidegger D . Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747