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 FO(骨髓瘤细胞)
  • FO(骨髓瘤细胞)
  • FO(骨髓瘤细胞)

    参考价格: ¥0.00
    品  牌:ATCC
    产品型号:CRL-1646 (株)
    所在地区:上海
    上架时间:2012年08月01日
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    FO(骨髓瘤细胞)

    FO(骨髓瘤细胞)
     
    Cell Biology
    ATCC® Number: CRL-1646™ Price: $255.00
    Designations:
    FO
    Depositors:
    SF de St. Groth
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    suspension
    Organism:
    Mus musculus (mouse)
    Morphology:
    lymphoblast
     
    Source:
    Cell type: B lymphoblast
    Disease: plasmacytoma; myeloma
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Antigen Expression:
    H-2d
    Strain:
    BALB/c
    Comments:
    The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
    The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
    The line can be used a fusion partner for making B cell hybridomas.
    Tested and found negative for ectromelia virus (mousepox).
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml.
    Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
    Medium renewal: Every 2 to 3 days
    Preservation:
    culture medium 95%; DMSO, 5%
    Doubling Time:
    8 to 10 hrs
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
    recommended serum: ATCC 30-2020
    References:
    1092: de St.Groth SF , Scheidegger D . Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747