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 C6(胶质瘤细胞)
  • C6(胶质瘤细胞)
  • C6(胶质瘤细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CCL-107 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    C6(胶质瘤细胞)

    C6(胶质瘤细胞)
     
    Cell Biology
    ATCC® Number: CCL-107™ Price: $203.00
    Designations:
    C6
    Depositors:
    G Sato
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Rattus norvegicus (rat)
    Morphology:
    fibroblast
     
    Source:
    Organ: brain
    Cell type: glial cell
    Disease: glioma
    Cellular Products:
    S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    Receptors:
    glucocorticoid
    Virus
    Susceptibility:
    vesicular stomatitis (Indiana); vaccinia; herpes simplex
    Virus Resistance:
    poliovirus 3
    Reverse Transcript:
    negative
    Cytogenetic
    Analysis:
    Stemline number is diploid. Karyotype is stable within the stemline number and is that of a normal male.
    Three cells with breaks; one with a secondary constriction, one with a dicentric, one with a rearrangement and four with terminal or centromere associations.
    Comments:
    The glial cell strain, C6, was cloned from a rat glial tumor induced by N-nitrosomethylurea by Benda et al. after a series of alternate culture and animal passages [PubMed: 4873531]. S-100 production increases ten fold as cells grow from low density to confluency.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
    Temperature: 37.0C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing:
    Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37C.


    Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

    Medium renewal: 2 to 3 times per week
    Preservation:
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004
    recommended serum: ATCC 30-2020
    recommended serum: ATCC 30-2040
    0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++): ATCC 30-2101
    Cell culture tested DMSO: ATCC 4-X
    References:
    1022: Benda P , et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370-371, 1968. PubMed: 4873531
    25965: Lightbody JJ , et al. Establishment of differentiated clonal strains of glial brain cells in culture. Fed. Proc. 27: 720, 1968.
    32720: Chen Y , et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005