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 A549 [A-549](肺癌细胞)
  • A549 [A-549](肺癌细胞)
  • A549 [A-549](肺癌细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CCL-185 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    A549 [A-549](肺癌细胞)

    A549 [A-549](肺癌细胞)
     
    Cell Biology
    ATCC® Number: CCL-185™ Price: $203.00
    Designations:
    A549
    Depositors:
    M Lieber
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Homo sapiens (human)
    Morphology:
    epithelial
    A549 [A-549](肺癌细胞)
     
    Source:
    Organ: lung
    Disease: carcinoma
    Cellular Products:
    keratin
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation:
    Isolation date: 1972
    Applications:
    Reverse Transcript:
    negative
    DNA Profile
    (STR):
    Amelogenin: X,Y
    CSF1PO: 10,12
    D13S317: 11
    D16S539: 11,12
    D5S818: 11
    D7S820: 8,11
    THO1: 8,9.3
    TPOX: 8,11
    vWA: 14
    Cytogenetic
    Analysis:
    This is a hypotriploid human cell line with the modal chromosome number of 12, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies.
    Isoenzymes:
    G6PD, B
    Age:
    58 years
    Gender:
    male
    Ethnicity:
    Caucasian
    Comments:
    This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. [23218]
    Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway. [58030]
    The cells are positive for keratin by immunoperoxidase staining.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing:
    Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
      Cultures can be established between 2 X 10(3) and 1 X 10(4) viable cells/cm2. Do not exceed 7 X 10(4) cels/cm2.
    6. Incubate cultures at 37°C.


    Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
    Interval: Maintain cultures at a cell concentration between 6 X 10(3) and 6 X 10(4) cell/cm2.

    Medium renewal: 2 to 3 times per week
    Preservation:
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time:
    about 22 hours
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004
    recommended serum: ATCC 30-2020
    References:
    23218: Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
    27669: Mayr GA , Freimuth P . A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365
    27819: Goodrum FD , Ornelles DA . The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383
    32299: St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830
    32347: Horikami SM , et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522
    32351: Huang S , et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475
    32373: Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
    32394: Fang R , Aust AE . Induction of ferritin synthesis in human lung epithelial cells treated with crocidolite asbestos. Arch. Biochem. Biophys. 340: 369-375, 1997. PubMed: 9143343
    32488: Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241
    32496: Evdokiou A , Cowled PA . Tumor-suppressive activity of the growth arrest-specific gene GAS1 in human tumor cell lines. Int. J. Cancer 75: 568-577, 1998. PubMed: 9466658
    32511: Giavedoni LD , Yilma T . Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J. Virol. 70: 2247-2251, 1996. PubMed: 8642649
    32514: Bartz SR , et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659
    32722: Garofalo R , et al. Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J. Virol. 70: 8773-8781, 1996. PubMed: 8971006
    32758: Jamaluddin M , et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674
    33091: Lewis JA , et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694
    58030: Lieber M , et al. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17: 62-70, 1976. PubMed: 175022