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 MC3T3-E1 Subclone 14(小鼠原成骨细胞)
  • MC3T3-E1 Subclone 14(小鼠原成骨细胞)
  • MC3T3-E1 Subclone 14(小鼠原成骨细胞)

    参考价格: ¥0.0000
    品  牌:ATCC
    产品型号:CRL-2594 (株)
    所在地区:上海
    上架时间:2012/8/1 4:30:01
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    MC3T3-E1 Subclone 14(小鼠原成骨细胞)

    MC3T3-E1 Subclone 14(小鼠原成骨细胞)
     
    Cell Biology
    ATCC® Number: CRL-2594™ Price: $255.00
    Designations:
    MC3T3-E1 Subclone 14
    Depositors:
    RT Franceschi
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Mus musculus (mouse)
    Morphology:
    fibroblast
     
    Source:
    Organ: bone
    Tissue: calvaria
    Cell type: preosteoblast
    Cellular Products:
    collagen [51540]
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Tumorigenic:
    Yes, in immunodeficient mice
    (forms bone-like ossicles)
    Strain:
    C57BL/6
    Age:
    newborn
    Comments:
    A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line.
    The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid.
    The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. [51540]
    They form a well mineralized extracellular matrix (ECM) after 10 days.
    The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14. [51540]
    Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor. [51540]
    Subclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor. [51540]
    After implantation into immunodeficient mice, highly differentiating subclones form bone-like ossicles resembling woven bone, while poorly differentiating cells only produce fibrous tissue. [51540]
    These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine and 1 mM sodium pyruvate, but without ascorbic acid (GIBCO, Custom Product, Catalog No. 0010083DJ). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%
    Temperature: 37.0C
    Subculturing:
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

    Subcultivation ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

    Medium renewal: Every 2 to 3 days
    Preservation:
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products:
    recommended serum: ATCC 30-2020
    derived from same cell line: ATCC CRL-2593
    derived from same cell line: ATCC CRL-2595
    derived from same cell line: ATCC CRL-2596
    References:
    51540: Wang D , et al. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J. Bone Miner. Res. 14: 893-903, 1999. PubMed: 10352097