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 A10(大鼠主动脉血管平滑肌细胞)
  • A10(大鼠主动脉血管平滑肌细胞)
  • A10(大鼠主动脉血管平滑肌细胞)

    参考价格: ¥0.00
    品  牌:ATCC
    产品型号:CRL-1476 (株)
    所在地区:上海
    上架时间:2012年08月01日
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    A10(大鼠主动脉血管平滑肌细胞)

    A10(大鼠主动脉血管平滑肌细胞)
     
    Cell Biology
    ATCC® Number: CRL-1476™ Price: $203.00
    Designations:
    A-10
    Depositors:
    W Carlisle
    1
    Shipped:
    frozen
    Medium & Serum:
    Growth Properties:
    adherent
    Organism:
    Rattus norvegicus (rat)
    Morphology:
    myoblast
    A10(大鼠主动脉血管平滑肌细胞)
     
    Source:
    Organ: aorta, thoracic
    Tissue: medial layer
    Cellular Products:
    myokinase; creatine phosphokinase; myosin
    Permits/Forms:
    In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Applications:
    Strain:
    DB1X
    Age:
    embryo
    Comments:
    The clonal cell line A10 was derived by B. Kimes and B. Brandt from the thoracic aorta of DB1X embryonic rat and possesses many of the properties characteristic of smooth muscle cells.
    The cells produce spontaneous action potentials at the stationary phase of the growth cycle and exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase.
    Propagation:
    ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0C
    Subculturing:
    Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.


    Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

    Medium renewal: Every 3 to 4 days
    Preservation:
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time:
    29 hours
    Related Products:
    Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
    recommended serum: ATCC 30-2020
    References:
    1061: Kimes BW , Brandt BL . Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301
    32281: Zhang X , et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783
    32468: Gordon EM , et al. Factor XII-induced mitogenesis is mediated via a distinct signal transduction pathway that activates a mitogen-activated protein kinase. Proc. Natl. Acad. Sci. USA 93: 2174-2179, 1996. PubMed: 8700904
    32530: Zhang X , Smith CD . Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761