名称：正品原装| hCD4+/45RA- T Cell Column 4 PK
Store all reagents at 2 - 8° C.
Designed to isolate sub-populations of CD4+/CD45RA- human T cells via high affinity negative selection. This may be achieved by warming the 10X column buffer bottle in a 37° C water bath for 5 - 10 mintues. The resulting column eluate is a highly enriched T cell subset population with minimal contamination of either CD45RA+ or CD8+ cells. Process cells on a density gradient, like Ficoll Hypaque, to enrich for mononuclear cells.
Principle of Selection
Leukocyte suspensions are incubated with a mixture of monoclonal antibodies and then loaded onto T Cell Subset Columns. Should this be the case, do not carry out the 1:10 buffer dilution (as indicated in step #1) until all salts are in solution. B cells and non-selected T cell subsets bind to anti-Ig coated glass beads via F(ab)-surface immunoglobulin (Ig) interactions, while monocytes bind to Ig coated glass beads via Fc interactions.