名称：正品订购| hIL-12 Rb1 Fluor MAb (C 69310)
Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C. Washed cells are incubated with the fluorescein-labeled monoclonal antibody that binds to cells expressing IL-12 Rβ1. Unbound fluorescein-conjugated antibody is then washed from the cells.
Designed to quantitatively determine the percentage of cells bearing the IL-12 Rβ1 chain within a population and qualitatively determine the density of the IL-12 Rβ1 on cell surfaces by flow cytometry. Whole blood should be collected in evacuated tubes containing EDTA or heparin as the anticoagulant.
Principle of the Test
Cells expressing IL-12 Rβ1 are fluorescently stained, with the intensity of staining directly proportional to the density of IL-12 Rβ1. Cultures: Continuous cell lines or activated cell cultures should be centrifuged at 500 x g for 5 minutes and washed three times in an isotonic PBS buffer (supplemented with 0.5% BSA), Cell surface expression of IL-12 Rβ1 is determined by flow cytometric analysis using 488 nm wavelength laser excitation.