名称：正品订购| hIL-17E Fluor MAb (Cl 182203)
Storage: Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C.
Flow cytometric analysis of fluorescein conjugates will generate a signal that can be detected. The reagent is designed for flow cytometric applications intended to identify and quantitate cells expressing cytoplasmic forms of the protein recognized by the monoclonal. Cells are initially subjected to a fixation step in order to minimize leakage of proteins out of the cell.
Principle of the Test
Fixed cells are permeabilized, allowing conjugated antibodies access to proteins within the cell. Prepare a sterile solution containing 0.1% (w/v) saponin, 0.05% (w/v) NaN3 in Hanks’ Balanced Salt Solution (HBSS). Store at room temperature for no longer than 1 month. The conjugated antibody is allowed to penetrate and bind to its target within the cell. Following a final wash, cells are analyzed on a flow cytometer.