名称：原装供应| Nru I from Nocardia rubra
规格：1,000 U (10 U/µl)
Source: From Nocardia rubra
Incubation temperature: +37°C
Sequence specificity: Nru I recognizes the sequence T*CG↓°CG*A and generates fragments with blunt ends. The amount of radioactivity released is calculated as a percentage of input radioactivity, then divided by the number of units of enzyme. That number is stated under "Exo" (on the product label).
Heat inactivation: Nru I can be heat inactivated by incubating it for 15 minutes at +65°C.
Compatible ends: Nru I ends are compatible with any blunt end.
Approx. 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Nru I for 4 h at 37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithioerythritol, pH approx. 7.5.
Nru I is an isoschizomer of Spo I.
Methylation sensitivity: Nru I is inhibited by overlapping dam methylation at the sites indicated (*) on the recognition sequence. Depending on its position, the presence of 5-methylcytosine may either inhibit (*) or have no effect (°).