名称：原装供应| Human CCL5/RANTES Quantikine ELISA Kit
SAMPLE COLLECTION AND STORAGE
Plasma - Collect plasma on ice using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 2 - 8° C at 1000 x g within 30 minutes of collection. An additional centrifugation step of the plasma at 10,000 x g for 10 minutes at 2 - 8° C is recommended for complete platelet removal.
Cell Culture Supernates - Remove particulates by centrifugation and assay immediately or aliquot and store samples at ≤ -20° C. Avoid repeated freeze-thaw cycles. The Stop Solution provided with this kit is an acid solution. Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.
Calibrator Diluent RD6-11 contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes at room temperature before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at ≤ -20° C. Avoid repeated freeze-thaw cycles.