名称：正品原装| Mouse CD3+ T Cell Enrichment Column, Large
Store at 2 - 8 °C. DO NOT FREEZE.
Designed to prepare purified murine T cell populations via high affinity negative selection. The supernatant is decanted and the cells resuspended in the appropriate culture medium. The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents. Store at 2 - 8 °C. DO NOT FREEZE. The collected cells are centrifuged at 250 x g for 5 minutes.
Principle of Selection
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns. B cells bind, via F(ab)-surface Immunoglobulin (Ig) interactions, to glass beads coated with anti-Ig while monocytes bind, via Fc interactions, to the glass beads coated with Ig. The eluate can be collected in a waste receptacle.
The column is now ready to be loaded with cells. Load the remaining 5 mL of cell suspension onto the column and let the column incubate 5 minutes further at room temperature. The resulting column eluate contains highly enriched T cell populations.