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 Mouse CD3+ T Cell Enrichment Column, Large
  • Mouse CD3+ T Cell Enrichment Column, Large
  • Mouse CD3+ T Cell Enrichment Column, Large

    参考价格: ¥2270.0000
    品  牌:R&D
    产品型号:MTCC-2000 (1 CL)
    所在地区:上海
    上架时间:2012/9/27 9:20:01
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    Mouse CD3+ T Cell Enrichment Column, Large
    名称:正品原装| Mouse CD3+ T Cell Enrichment Column, Large
    货号:MTCC-2000
    产地:R&D
    规格:1 CL
    价格:2270
    Store at 2 - 8 °C. DO NOT FREEZE.
    Intended Use
    Designed to prepare purified murine T cell populations via high affinity negative selection. The supernatant is decanted and the cells resuspended in the appropriate culture medium. The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents. Store at 2 - 8 °C. DO NOT FREEZE. The collected cells are centrifuged at 250 x g for 5 minutes.
    Principle of Selection
    Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns. B cells bind, via F(ab)-surface Immunoglobulin (Ig) interactions, to glass beads coated with anti-Ig while monocytes bind, via Fc interactions, to the glass beads coated with Ig. The eluate can be collected in a waste receptacle.
    The column is now ready to be loaded with cells. Load the remaining 5 mL of cell suspension onto the column and let the column incubate 5 minutes further at room temperature. The resulting column eluate contains highly enriched T cell populations.