名称：正品原装| mCD4+ T Cell Column, Sm 4 PK
Designed to prepare sub-populations of CD4 mouse T cells via high affinity negative selection. The collected cells are centrifuged at 250 x g for 5 minutes. The resulting column eluate is a highly enriched T cell subset population with minimal depletion of the selected T cells. The cells are then washed twice with 10 mL of 1X Column Buffer, spinning the cells at 300 x g for 10 minutes and decanting supernatant after each wash.
Principle of Selection
Leukocyte suspensions are incubated with a mixture of monoclonal antibodies and then loaded onto T Cell Subset Columns. The waste receptacle is replaced with a sterile 15 mL conical centrifuge tube. B cells, non-selected T cells and monocytes bind to glass beads coated with anti-immunoglobulin via both F(ab) and Fc interactions. The final cell pellet is resuspended in 2 mL of 1X Column Buffer. The resulting column eluate contains a highly enriched T cell subset population with virtually no B cells, monocytes, or non-selected T cells.