上海叶舟生物科技有限公司

 mCD4+ T Cell Column, Sm 4 PK
  • mCD4+ T Cell Column, Sm 4 PK
  • mCD4+ T Cell Column, Sm 4 PK

    参考价格: ¥3720.0000
    品  牌:R&D
    产品型号:MCD4C-1000 (1 KT)
    所在地区:上海
    上架时间:2012/9/27 9:20:01
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    mCD4+ T Cell Column, Sm 4 PK
    名称:正品原装| mCD4+ T Cell Column, Sm 4 PK
    货号:MCD4C-1000
    产地:R&D
    规格:1 KT
    价格:3720
    Intended Use
    Designed to prepare sub-populations of CD4 mouse T cells via high affinity negative selection. The collected cells are centrifuged at 250 x g for 5 minutes. The resulting column eluate is a highly enriched T cell subset population with minimal depletion of the selected T cells. The cells are then washed twice with 10 mL of 1X Column Buffer, spinning the cells at 300 x g for 10 minutes and decanting supernatant after each wash.
    Principle of Selection
    Leukocyte suspensions are incubated with a mixture of monoclonal antibodies and then loaded onto T Cell Subset Columns. The waste receptacle is replaced with a sterile 15 mL conical centrifuge tube.  B cells, non-selected T cells and monocytes bind to glass beads coated with anti-immunoglobulin via both F(ab) and Fc interactions. The final cell pellet is resuspended in 2 mL of 1X Column Buffer. The resulting column eluate contains a highly enriched T cell subset population with virtually no B cells, monocytes, or non-selected T cells.