名称：正品原装| Mouse Sca-1+ Stem Cells PlusCellect
Reagents are stable for 12 months from the date of receipt when stored in the dark at 2 - 8° C. DO NOT FREEZE. Purity of recovered Sca-1+ cells typically ranges between 65 - 85%.
The Mouse Sca-1+ PlusCellect Kit is designed to directly isolate stem/progenitor cells from mouse bone marrow via a positive selection principle. Keep the biotinylated antibody and ferrofluid incubation times the same. After filtration, the cells can be used directly for selection. Or, if desired, the red blood cells (RBC) can be removed. The resulting cell preparation is highly enriched for Sca-1+ cells.
The PE-conjugated detection antibody provided in this kit contains sodium azide which may react with lead and copper plumbing to form explosive metallic azides. Use sterile equipment when pipetting reagents that will be reused at a later date. Flush with large volumes of water during disposal. Harvest the hind leg bones of mice* and prepare a mononuclear suspension of bone marrow cells using traditional methods.