名称：正品订购| h/mIL-12 Rb2 A488 MAb (305719)
Reagents are stable for twelve months from the date of receipt when stored in the dark at 2° - 8° C. The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Designed to quantitatively determine the percentage of cells bearing IL-12 Rβ2 within a population and qualitatively determine the density of IL-12 Rβ2 on cell surfaces by flow cytometry. The purified antibody was then conjugated to Alexa Fluor®488 fluorochrome. Cell surface expression of IL-12 Rβ2 is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515 - 545 nm.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, The expression of IL-12 Rβ2 is absent on naïve T cells. Activation of T cells via TCR up-regulates IL-12 Rβ2 expression on human Th1 cells, but not Th2 cells. NS0-derived, recombinant human Interleukin 12 Receptor beta 2 extracellular domain (rhIL-12 Rβ2; aa 24 - 622; Accession # Q99665).