名称：正品订购| hIL-10 R bet PE MAb (Cl 90220)
Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C. Cell surface expression of IL-10 Rβ is determined by flow cytometric analysis using 488 nm wavelength laser excitation.
Designed to quantitatively determine the percentage of cells bearing IL-10 Rβ within a population and qualitatively determine the density of IL-10 Rβ on cell surfaces by flow cytometry. Unbound PE-conjugated antibody is then washed from the cells. Cells expressing IL-10 Rβ are fluorescently stained, with the intensity of staining directly proportional to the density of IL-10 Rβ.
Principle of the Test
As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Washed cells are incubated with the PE-labeled monoclonal antibody that binds to the cells expressing IL-10 Rβ. Cells should then be resuspended in the same buffer to a final concentration of 4 x 106 cells/mL and 25 μL of cells (1 x 105) are transferred to a 5 mL tube for staining.