名称：正品订购| hIL-19 PE MAb (Cl 152112)
Storage: Reagents are stable for twelve months from date of receipt when stored in the dark at 2° - 8° C.
Principle of the Test
Fixed cells are permeablized, allowing conjugated antibodies access to proteins within the cell. The conjugated antibody is allowed to penetrate and bind to its target within the cell. Following a final wash, Cells are centrifuged at 200 x g for 7 minutes. cells are analyzed on a flow cytometer. saponin must be included in all buffers used (i.e. both the staining and washing steps).
The reagent is designed for flow cytometric applications intended to identify and quantitate cells expressing cytoplasmic and membrane bound forms of human IL-19. Wash the cells one time in PBS by centrifugation at 200 x g for 7 minutes. Cells are initially subjected to a fixation step in order to minimize leakage of proteins out of the cell.