名称：现货供应|PD98059 (MEK1 Inhibitor)
PD98059 (MEK1 Inhibitor) is soluble in DMSO at 10-100 mM. For use with in vitro or cell-based assays, it may be diluted into aqueous buffers to yield desired concentrations (<100 µM) with final DMSO concentrations of ~0.1%. PD98059 (MEK1 Inhibitor) has been shown to act in vivo as a highly selective inhibitor of MEK1 activation and the MAP kinase cascade. This product is for in vitro research use only and is not intended for use in humans or animals. IC50 values for inhibitory activity against MEK1 are around 5-10 µM.
Directions for Use
For experiments with cultured cells, CST recommends pretreating with this inhibitor for one hour prior to stimulation. Supplied as a lyophilized yellow powder. MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. For 20 mM stock, reconstitute 1.5 mg in 280 µl DMSO (5 mg in 933.3 μl). For 50 mM stock, reconstitute 1.5 mg in 112 µl DMSO (5 mg in 373.3 μl). Store in aliquots at -20ºC. See enclosed MSDS for further information.