名称：原装进口| Phospho-Stat1 (Tyr701) Blocking Peptide
Directions for Use
For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 μl total volume. In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide.
This peptide is used to block Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 reactivity. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. Recommended antibody dilutions can be found on the product data sheet.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols.