规格: 20 RXN
名称: BL21 STAR DE3 PLYSS COMBO
E. coli RNA polymerases, transcription from the T7 promoter is uncoupled to translation in E. coli. This results in mRNA transcripts unprotected by ribosomes, which are then subject to enzymatic degradation
by endogenous RNases (1). The reduced level of transcripts in the cell often leads to greatly reduced levels of protein yield (Figure1). The BL21 Star? strains contain a mutation in the gene encoding
RNaseE (rne131), which is one of the major sources of this mRNA degradation (2). BL21 Star? cells significantly improve the stability of mRNA transcripts and increase protein expression yield from T7
promoter-based vectors (3) (Figure 2).
BL21 Star? E. coli strains are high-performance BL21 hosts designed for improving protein yield in a T7 promoter-based expression system. Because T7 RNA polymerase synthesizes mRNA more rapidly than