名称: 原装|Recombinant Human Protein Kinase B
Physical Appearance Sterile Filtered clear solution.
Formulation AKT1 (0.1mg/ml) is supplied in 50mM Tris pH 7.5, 0.15M NaCl, 0.27M sucrose,10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free.
Unit Definition 250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30°C with a final ATP concentration of 100µM. The optimal concentration should be determined for each specific application.
Please note :Kinase activity may vary depending on the substrate and reaction conditions.
Storage Conditions Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C- -80°C for longer periods of time. Avoid multiple freeze-thaw cycles.