规格: 20 UG EACH
名称: DESPAC5.1/V5-HIS A-B-C KIT
The DES?-Constitutive Kit contains 20 μg each of a selection vector (pCoBlast or pCoHygro), pAc5.1/V5-His A, B, C, and pAc5.1/V5-His/lacZ. It also contains the Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO? Schneiders Drosophila Medium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
The DES?-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin