规格: 1 KIT
名称: FluoSpheres® Size Kit #1, Carboxylate-modified Microspheres, Red Fluorescent (580⁄605) *six sizes, 1 mL each* *2% Solids*
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres® Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0 μm sizes.
FluoSpheres® Microsphere Specifications
Label (Ex/Em): Red fluorescent (580/605)
Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0 μm
Coupling surface: Carboxylate
Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)
Characteristics of the Various FluoSpheres® Coupling Surfaces
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
Aldehyde—sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.