规格: 1-column kit
名称: SulfoLink Immobilization Kits and Coupling Resin
Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.
When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.
Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
Specific conjugation through sulfhydryl (-SH) groups – The iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Separate kits optimized for peptides or proteins – Kits include optimized reagents for preparing peptide or protein samples for efficient immobilization
Fast – Spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions – Use pH 7.5-9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine•HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity – Immobilize 1-2 mg peptide or 2-20 mg protein per 2-ml column of SulfoLink Coupling Resin.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.