名称: Citraconic Anhydride
Thermo Scientific Pierce Citraconic Anhydride reacts with primary amines to to form an amide bond with a terminal carboxyl group. This modification can be reversed using acidic conditions (pH 3-4) or with hydroxylamine.
Citraconic Anhydride (2-methylmaleic anhydride) is used to block primary amine groups at alkaline pH values (pH 7-9). At acidic pH (3-4), the amide linkage is hydrolyzed and citraconic acid is released, revealing the original amine.
Reactive toward: Primary amines
Chemical name: 2-methylmaleic anhydride
CAS number: 616-02-4
Chemical formula: C5H4O3
Molecular weight: 112.08
Form: Colorless to slightly yelllow, clear liquid
Temporarily block amines to derivatize other parts of the molecule
Deblock amines using acidic conditions or hydroxylamine
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.