规格: 96 assays
名称: H2A.X (Ser139) Dual Detect CELISA ASSAY Kit (Fluorogenic Detection)
Product Overview: Dual-Detect CELISA is a simple and efficient cell-based ELISA assay to measure the relative expression levels of both the total and the phosphorylated form of H2A.X in whole cells in a 96 well microplate format using dual fluorescent detection. This allows for the normalization of total protein between wells, thus allowing a more accurate measurement of phosphorylation levels among different cells and/or growth conditions. Cells are cultured directly in the microplate and stimulated as desired. They are then fixed and simultaneously incubated with two primary antibodies. One is a phosphospecific antibody directed against the phosphorylated epitope that correlates with H2A.X activation and the other is a total antibody that recognizes the H2A.X target protein, regardless of phosphorylation status. Both the total target protein and the phosphorylated protein are measured in a single well using a double immunoenzymatic labeling procedure, including both horseradish-peroxidase (HRP) and alkaline phosphatase (AP), and two spectrally distinct fluorogenic substrates for HRP and AP. The fluorescence of the phosphorylated protein is normalized to the total protein in each well, thus reducing well-to-well variations.
Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.