规格: 2 ml
名称: Agarose bound Phaseolus vulgaris Erythroagglutinin (PHA-E)
Agarose bound* Phaseolus vulgaris Erythroagglutinin (PHA-E) is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions.
Our agarose bound lectins are supplied at a constant concentration of lectin per ml of gel. The concentration at which each lectin is prepared is selected in order to achieve the highest glycoconjugate binding capacity per mg of lectin present in the gel.
The technique we have developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure.