规格: 50 assays
名称: MDR1 Shift Assay 原装正品
Application Notes: The Millipore MDR1 Shift Assay provides a convenient method to probe conformational changes in MDR1 that occur upon transport of MDR1 substrates. Thus, the kit may be used to determine the extent to which small molecules act as transport substrates for MDR1. Because UIC2 binding is drastically increased in the presence of MDR1 substrates, the MDR1 Shift Assay can be utilized for improved, highly sensitive and specific detection of MDR1 expression. In addition, the kit is valuable for the study of structure-function relationships in cells expressing mutant MDR1 molecules.
The kit includes the UIC2 antibody, a conformation-sensitive mouse monoclonal against human MDR1, and an isotype-matched negative control mouse IgG2a. A phycoerythrin-conjugated anti-mouse IgG is provided for sensitive detection of bound primary antibody to the cells. In addition, a potent transport substrate of MDR1, vinblastine, and the diluent control (DMSO) are provided as positive and negative controls for inducing the conformational change in MDR1. Concentrated PBS (10x) and a BSA solution are included as components for medium for washing the cells after antibody binding steps. Also included is a propidium iodide solution to stain dead cells and exclude them from analysis.
Components: Anti-MDR1 Monoclonal UIC2: (Part no. 90291) - One vial containing 250 μl of 1 mg/ml UIC2 mouse monoclonal anti-human MDR1, sterile-filtered in PBS without preservatives. Mouse IgG2a Isotype Control: (Part no. 90292) - One vial containing 125 ul of 1 μg/ml negative control mouse IgG2a monoclonal antibody, sterile-filtered in PBS without preservatives.
Phycoerythrin-Labeled Anti-Mouse IgG: (Part no. 90354) - One vial containing R-phycoerythrin-labeled anti-mouse IgG dried from 190 μl buffered solution containing BSA as a stabilizer and sodium azide as a preservative.
Vinblastine Stock Solution: (Part no. 90295) - One vial containing 50 μl of 22 mM vinblastine in DMSO. Note DMSO solidifies at 4C, so thawing is recommended before use.
DMSO: (Part no. 90294) - One vial containing 500 μl of DMSO. Warm to room temperature before extracting and using as DMSO solidifies at cold temperatures.
Sterile 10x PBS: (Part no. 90297) - One bottle containing 100 ml of sterile-filtered 10x PBS.
Sterile 30% BSA: (Part no. 90303) - One bottle containing 35 ml of sterile-filtered 30% BSA in PBS.
Propidium Iodide Solution: (Part no. 90296) - One vial containing 1.5 ml of 50 μg/ml propidium iodide in PBS.