规格: 10 PACK
名称: 1-5ML 7K MW Cassettes (10PACK)
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7,000 daltons.
Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.
The cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Easy to use – no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis – flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery – rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Three cassette sizes – select the cassette that best suits your sample volume
Color-coded – green cassette frame specifies cassettes with the 7,000 MW pore size membrane
7K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30µm)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 13%
Sulfur Content: 0.1 to 0.15%
Heavy Metals Content: Trace
Characterization of 7K membrane performance. Dialysis membrane for Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) were evaluated with the following protein samples: vitamin B12 (1.4 kDa) insulin chain B (3.5 kDa), insulin (5 kDa), and cytochrome C (12.5 kDa). The samples (1mg/mL) in either 0.15M sodium chloride or 0.2M carbonate-bicarbonate buffer pH 9.4 were dialyzed overnight (16 hours) at room temperature in a rotating cell. The retentate concentration of protein was estimated by absorbance at 280nm (360nm for vitamin B12). Results indicate that the effective membrane pore size is approximately 7K.