上海浩洋生物科技有限公司

 DreamTaq PCR Master Mix (2X)
  • DreamTaq PCR Master Mix (2X)
  • DreamTaq PCR Master Mix (2X)

    参考价格: ¥515.00
    品  牌:MBI
    产品型号:K1071 (200 react)
    所在地区:上海
    上架时间:2013年03月13日
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    DreamTaq PCR Master Mix (2X)
    产品名称:DreamTaq PCR Master Mix (2X)
    规格:200 react
    货号:K1071
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    Definition of Activity Unit One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
    Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl2, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/mL BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/mL [3H]-dTTP.
    Highlights
    Robust amplification with minimal optimization
    High yields of PCR products
    Higher sensitivity compared to conventional Taq DNA polymerase
    Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
    Incorporates modified nucleotides, but does not incorporate dUTP
    Applications
    Routine PCR amplification of DNA fragments up to 6 kb
    High throughput PCR
    Genotyping
    Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq buffer, which includes 20 mM MgCl2.
    MBI K1071 DreamTaq™ PCR Master Mix (2X) 200 react 515
    MBI K1072 DreamTaq™ PCR Master Mix (2X) 1000 react 2060