Storage Condition -20 C
Compatible Ends BbvCI, Bpu10I, Bpu1102I, HpyF3I.
Conditions for 100% Activity 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
Incubate at 37°C
Digestion of agarose-embedded DNA Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Storage Buffer Eco81I is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.
Superior quality-stringent quality control and industry leading manufacturing process
Convenient color-coded Five Buffer System
Includes universal Tango buffer for double-digestions
BSA premixed in reaction buffers
Wide selection of restriction endonuclease specificities
Restriction site mapping
Restriction fragment length polymorphism (RFLP)
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.