Formulation 1 µL of enzyme (1 FDU) cleaves 1 μg of pTZ19RJL2 DNA-BseLI fragments in 5 minutes at 37°C in 1X FastDigest Buffer.
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Recommended Reaction Conditions 1X FastDigest buffer or 1X FastDigest Green buffer at 37°C.
1 µL of FastDigest NotI is formulated to digest up to:
1 µg of pTZ19RJL2 DNA/BseLI fragments in 5 minutes
1 µg of plasmid DNA in 30 minutes
0.2 µg of PCR product in 5 minutes
1 µg of genomic DNA in 10 min or 5 µg of genomic DNA in 60 minutes
100% activity of all FastDigest enzymes in the universal buffer
100% buffer compatibility with downstream applications
Complete digestion in 5-15 minutes
Direct loading on gels
No star activity
176 FastDigest enzymes available
Restriction site mapping
Restriction fragment length polymorphism (RFLP)