产品名称：Terminal Deoxynucleotidyl Transferase
Storage Condition -20 C
5X Reaction Buffer 1 M potassium cacodylate, 125 mM Tris, 0.05% (v/v) Triton X-100, 5 mM CoCl2 (pH 7.2 at 25°C)
Concentration 20 U/µL
Definition of Activity Unit
One unit of the enzyme catalyzes the incorporation of 1 nmol of deoxythymidylate into a polynucleotide fraction (adsorbed on DE-81) in 60 min at 37°C.
Enzyme activity is assayed in the following mixture: 200 mM potassium cacodylate (pH 7.2), 1 mM CoCl2, 0.01% (v/v) Triton X-100, 10 µM oligo(dT)10, 1 mM dTTP, and 0.4 MBq/mL [3H]-dTTP.
Inactivation Inactivated by heating at 70°C for 10 min or by addition of EDTA.
Inhibition Inhibitors: metal chelators, ammonium, chloride, iodide, phosphate ions
Quality Control The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests.
Source E.coli cells carrying a cloned gene encoding calf thymus terminal deoxynucleotidyl transferase.
Storage Buffer The enzyme is supplied in 100 mM potassium acetate (pH 6.8), 2 mM 2-mercaptoethanol, 0.01% (v/v) Triton X-100 and 50% (v/v) glycerol.
Incorporates modified nucleotides (e.g., fluorescein-, biotin-, aminoallyl-labeled nucleotides)
Production of synthetic homo- and heteropolymers (see? Reference 1)
Homopolymeric tailing of linear duplex DNA with any type of 3-OH terminus (see? References 2, 3)
Oligodeoxyribonucleotide and DNA labeling (see? References 2, 4-8)
5-RACE (Rapid Amplification of cDNA Ends) (see? Reference 9)
In situ localization of apoptosis (see? Reference 10)