上海浩洋生物科技有限公司

 Klenow Fragment, exo–
  • Klenow Fragment, exo–
  • Klenow Fragment, exo–

    参考价格: ¥1483.00
    品  牌:MBI
    产品型号:EP0422 (1500 units)
    所在地区:上海
    上架时间:2013年03月14日
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    Klenow Fragment, exo–
    产品名称:Klenow Fragment, exo–
    规格:1500 units
    货号:EP0422
    厂商:MBI
    产品介绍:
    Storage Condition -20 C
    Hazardous No
    10X Reaction Buffer 500 mM Tris-HCl (pH8.0 at 25°C), 50mM MgCl 2 , 10mM DTT.
    Definition of Activity Unit
    One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C, using poly(dA-dT)·poly(dA-dT) as a template·primer.
    Enzyme activity is assayed in the following mixture: 67mM potassium phosphate (pH7.4), 6.7mM MgCl 2 , 1mM 2-mercaptoethanol, 0.033mM dATP, 0.033mM dTTP, 0.4MBq/mL [ 3 H]-dTTP, and 62.5µg/mL poly(dA-dT)·poly(dA-dT).
    Inactivation Inactivated by heating at 75°C for 10min or by the addition of EDTA.
    Inhibition Inhibitors: metal chelators, PP i , P i (at high concentrations) ( see Reference 7).
    Molecular Weight 68kDa monomer.
    Highlights
    Lacks 3→5 exonuclease activity
    Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
    Active in restriction enzyme, PCR, and RT buffers
    Applications
    Random-primed DNA labeling ( see ? References 2-4)
    Labeling by fill-in 5-overhangs of dsDNA
    Strand displacement amplification (SDA) ( see ? Reference 5)
    DNA sequencing by the Sanger method ( see ? Reference 6)