上海浩洋生物科技有限公司

 293/mTLR9
  • 293/mTLR9
  • 293/mTLR9

    参考价格: ¥10304.0000
    品  牌:invivogen
    产品型号:293-mtlr9 (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013/3/14 15:21:45
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    293/mTLR9
    产品名称:293/mTLR9
    规格:5-7 x 10e6 cells
    货号:293-mtlr9
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: blasticidin
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    293/mTLR9 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR9 plasmid which expresses the murine TLR9 gene.
    293XL/TLR9A cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR9 plasmid which expresses the human TLR9 gene.
    1 vial containing 3-7 x 106 cells
    100 μl Blasticidin (10 mg/ml)
    1 ml Normocin™ (50 mg/ml)
    TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane [1]. TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation [2]. TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively [3]. TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded [4].