规格：5-7 x 10e6 cells
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Shipped on dry ice
293/mTLR9 cells were obtained by stable transfection of HEK293 cells with a pUNO-TLR9 plasmid which expresses the murine TLR9 gene.
293XL/TLR9A cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR9 plasmid which expresses the human TLR9 gene.
1 vial containing 3-7 x 106 cells
100 μl Blasticidin (10 mg/ml)
1 ml Normocin™ (50 mg/ml)
TLR9 is expressed in the endoplasmic reticulum of resting cells in contrast to most TLRs that are located on the plasma membrane . TLR9 recognizes specific unmethylated CpG-ODN sequences, that distinguish bacterial DNA from mammalian DNA. TLR9 engages an intracellular signaling pathway that involves MyD88 leading to NF-κB translocation . TLR9 recognizes different CpG motifs; the optimal sequences being GTCGTT and GACGTT for human TLR9 and murine TLR9 respectively . TLR9 binds directly to CpG DNA and recognizes specifically CpG DNA that is unmethylated and single stranded .