规格：5-7 x 10e6 cells
Antibiotic resistance: blasticidin
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Shipped on dry ice
293XL/TLR7 cells were obtained by stable transfection of HEK293XL cells with a pUNO-TLR7 plasmid which expresses the human or murine TLR7 gene.
1 vial containing 3-7 x 106 cells
100 μl Blasticidin (10 mg/ml)
1 ml Normocin™ (50 mg/ml)
TLR7, which is abundantly expressed in lung, placenta, spleen and PBL, is phylogenetically close to TLR8 and TLR9 . TLR7 recognizes small synthetic molecules such as loxoribine and R848, an imidazoquinoline compound . TLR7 signaling involves the MyD88-dependent signaling cascade and induces the production of IFN-α, TNF-α and IL-12. Recently, single-stranded RNA (ssRNA) was identified as the natural ligand of TLR7 [3, 4]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in PDC. TLR7 signaling is abrogated by chloroquine indicating that it is dependent on endosomal acidification.