产品名称：HEK-Blue hMD2-CD14 cells
规格：5-7 x 10e6 cells
Antibiotic resistance: hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Shipped on dry ice
HEK-Blue™ MD2-CD14 cells were obtained by co-transfection of the human MD-2 and CD14 genes and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells. The SEAP reporter gene is under the control of the IL-12 p40 promoter fused to five NF-κB and AP-1 binding sites. Levels of SEAP can be easily determined with QUANTI-Blue™, a detection medium that turns purple/blue in the presence of alkaline phosphatase. Alternatively, HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP can be used.