上海浩洋生物科技有限公司

 HEK-Blue hMD2-CD14 cells
  • HEK-Blue hMD2-CD14 cells
  • HEK-Blue hMD2-CD14 cells

    参考价格: ¥15952.00
    品  牌:invivogen
    产品型号:hkb-hmdcd (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013年03月15日
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    HEK-Blue hMD2-CD14 cells
    产品名称:HEK-Blue hMD2-CD14 cells
    规格:5-7 x 10e6 cells
    货号:hkb-hmdcd
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: hygromycin, Zeocin™
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    HEK-Blue™ MD2-CD14 cells were obtained by co-transfection of the human MD-2 and CD14 genes and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells. The SEAP reporter gene is under the control of the IL-12 p40 promoter fused to five NF-κB and AP-1 binding sites. Levels of SEAP can be easily determined with QUANTI-Blue™, a detection medium that turns purple/blue in the presence of alkaline phosphatase. Alternatively, HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP can be used.