上海浩洋生物科技有限公司

 HEK-Blue IFN-α/β cells
  • HEK-Blue IFN-α/β cells
  • HEK-Blue IFN-α/β cells

    参考价格: ¥14016.00
    品  牌:invivogen
    产品型号:hkb-ifnab (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013年03月15日
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    HEK-Blue IFN-α/β cells
    产品名称:HEK-Blue IFN-α/β cells
    规格:5-7 x 10e6 cells
    货号:hkb-ifnab
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: blasticidin, Zeocin™
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    1 vial containing 3-7 x 106 cells
    100 μl Blasticidin (10 mg/ml)
    100 μl Zeocin™ (100 mg/ml)
    1 ml Normocin™ (50 mg/ml)
    1 pouch of QUANTI-Blue™ (SEAP detection medium)
    HEK-Blue™ IFN-α/β cells allow the detection of bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT2 and IRF9 genes to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2 and STAT1) are naturally expressed in sufficient amounts. The cells were further transfected with a SEAP reporter gene under the control of the IFN-α/β inducible ISG54 promoter. Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IFN-α/β cells with recombinant human IFN-α can be blocked by anti-hIFN-α-IgA, a neutralizing monoclonal antibody.