上海浩洋生物科技有限公司

 HEK-Blue IL-4/IL-13 cells
  • HEK-Blue IL-4/IL-13 cells
  • HEK-Blue IL-4/IL-13 cells

    参考价格: ¥14016.00
    品  牌:invivogen
    产品型号:hkb-stat6 (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013年03月15日
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    HEK-Blue IL-4/IL-13 cells
    产品名称:HEK-Blue IL-4/IL-13 cells
    规格:5-7 x 10e6 cells
    货号:hkb-stat6
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: blasticidin, Zeocin™
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    1 vial containing 3-7 x 106 cells
    100 μl Blasticidin (10 mg/ml)
    100 μl Zeocin™ (100 mg/ml)
    1 ml Normocin™ (50 mg/ml)
    1 pouch of QUANTI-Blue™ (SEAP detection medium)
    Additionally provided with the HEK-Blue™ IL-4/IL-13 kit:
    100 μg anti-hIL-4 IgA antibody
    100 μg anti-hIL-13 IgA antibody
    HEK-Blue™ IL-4/IL-13 cells allow the detection of bioactive IL-4 and IL-13 by monitoring the activation of the STAT-6 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT6 gene to obtain a fully active STAT6 pathway. The other genes of the pathway are naturally expressed in sufficient amounts. The cells were further transfected with a STAT6-inducible SEAP reporter gene. HEK-Blue™ IL-4/IL-13 cells produce SEAP in response to IL-4 or IL-13 stimulation and to a lower extent IFN-α. The levels of SEAP secreted in the supernatant can be easily determined using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-4/IL-13 cells with human recombinant IL-4 or IL-13 can be blocked by the neutralizing monoclonal hIL-4-IgA and hIL-13-IgA antibodies, respectively.
    invivogen hkb-stat6 HEK-Blue™ IL-4/IL-13 cells 5-7 x 10e6 cells 14016
    invivogen hkb-stat6-kit HEK-Blue™ IL-4/IL-13 kit 1 kit 16144