上海浩洋生物科技有限公司

 HEK-Blue TGF-β cells
  • HEK-Blue TGF-β cells
  • HEK-Blue TGF-β cells

    参考价格: ¥14016.0000
    品  牌:invivogen
    产品型号:hkb-tgfb (5-7 x 10e6 cells)
    所在地区:上海
    上架时间:2013/3/15 13:28:27
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    HEK-Blue TGF-β cells
    产品名称:HEK-Blue TGF-β cells
    规格:5-7 x 10e6 cells
    货号:hkb-tgfb
    厂商:invivogen
    产品介绍:
    Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
    Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
    Guaranteed mycoplasma-free
    Shipped on dry ice
    Detects human TGF-β
    Detection range for human TGF-β: 0.1 - 10 ng/ml
    1 vial containing 3-7 x 106 cells
    100 μl Blasticidin (10 mg/ml)
    100 μl HygroGold™ (100 mg/ml)
    100 μl Zeocin™ (100 mg/ml)
    1 ml Normocin™ (50 mg/ml)
    1 pouch of QUANTI-Blue™ (SEAP detection medium)
    Additionally provided with the HEK-Blue™ TGF-β kit
    100 μg anti-hTGF-β IgA antibody
    HEK-Blue™ TGF-β cells allow the detection of bioactive TGF-β by monitoring the activation of the TGF-β/Smad pathway. They were generated by stable transfection of HEK293 cells with the human TGFBRI, Smad3 and Smad4 genes. They further express a Smad3/4-binding elements (SBE)-inducible SEAP reporter gene. Stimulation of HEK-Blue™ TGF-β cells with TGF-β induces the activation of the TGF-β/Smad signaling pathway leading to the formation of a Smad3/Smad4 complex. The heterocomplex enters the nucleus and binds SBE sites inducing the production of SEAP. The quantity of SEAP secreted in the supernatant can be easily assessed using QUANTI-Blue™. TGF-β-mediated SEAP production can be blocked using a neutralizing antibody, such as anti-hTGFβ-IgA