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现货供应Streck  Cell-Free RNA BCT游离RNA样本收集管
  • 现货供应Streck  Cell-Free RNA BCT游离RNA样本收集管
  • 现货供应Streck  Cell-Free RNA BCT游离RNA样本收集管

    产品报价: 12000
    品  牌:Streck
    产品型号:218975 218976
    适用范围:高教 职教
    所在地区:美国
    (联系我时,请说明是在教育装备采购网上看到的,谢谢!)
    详细说明

    现货供应Streck  Cell-Free RNA BCT游离RNA样本收集管

    现货供应Cell-FreeRNA BCT游离RNA样本收集管

    Streck218976

    【产品名称】Streck 218976 Cell-Free RNA BCT Blood Collection Tube血浆游离RNA采血管

    【Streck 218976 Cell-Free RNA BCT Blood Collection Tube无创管规格】

    抽血量 10.0mL

    抗凝血剂 K3EDTA

    添加剂 专有稳定剂

    使用前储存 室温保存

    包装规格 100管/盒

    Streck 液态活检系列产品

    名称

    规格

    货号

    Cell-Free DNA BCT

    6-Tube Pack(10ml)

    218961

    100-Tube Pack(10ml)

    218962

    6-Tube Pack(2ml)

    230242

    100-Tube Pack(2ml)

    230243

    Cell-Free RNA BCT

    6-Tube Pack

    218975

    100-Tube Pack

    218976

    Cell-Free DNA Urine Preserve

    20×5ml

    230216

    5×5ml

    230230

    使用说明

    Streck218976Cell-FreeRNA BCT®是一种直接全血采集管,用于收集,稳定和运输无细胞的血浆RNA。Streck218976Cell-FreeRNA BCT还稳定并保留白色的细胞RNA血细胞。该产品尚未被美国食品和药物管理局批准诊断使用。该产品仅供研究使用。不用于诊断程序。

    【STRECK 218976无创管的使用说明】

    美国Streck 218976 10mL Cell-Free RNA BCT Blood Collection Tube血浆游离RNA采血管是一种直接抽取全血的,可用于无细胞血浆RNA采集,稳定储存和运输的试管。STRECK 218976 Cell-Free RNA BCT Blood Collection Tube血浆游离RNA采血管能防止样品处理和贮存过程中的血细胞非目标背景的RNA的释放。注:本产品仅可用于科学研究,不可用于医学诊断。

    【STRECK 218976Cell-Free RNA BCT血浆游离RNA采血管 产品英文介绍】

    Streck 218976 Cell-Free RNA BCT Blood Collection Tube

    Cell-Free RNA BCT isa blood collection tube with a patented preservative which stabilizes cell-free RNA in plasma and prevents the release of non-target background RNA from blood cells during sample processing and storage.

    Cell-Free RNA BCT Blood Collection Tube reduces the need for immediate plasma preparation due to its unique stabilization properties. Cell-Free RNA is stable for up to 7 days at room temperature, allowing convenient sample collection, transport and storage.

    STRECK Cell-Free RNA BCT Catalog Number

    STRECKCell-Free RNA BCT 6-Tube Pack (RUO) 218975

    STRECKCell-Free RNA BCT 100-Tube Box (RUO)218976

    For Research Use Only. Not for use in diagnostic procedures in the U.S.

    BD Vacutainer® 静脉真空采血系统 -- 标本分析前处理系统产品

    BD Vacutainer® 静脉真空采血系统可用于在完全封闭状态采集、运输和处理临床科研及实验室所需的各种全血、血清或血浆标本。BD Vacutainer® 静脉真空采血管能有效减少标本的分析前误差,提高检测结果准确性,加强医院综合诊断治疗水平,严格控制血源性传染病的院内感染。同时减轻医务人员工作量,降低患者治疗成本,提高医院的综合效益。

    华雅再生医学 现货供应:美国Streck 218962 Cell-Free DNA BCTBlood Collection TubeDNA基因检测无创采血管 和美国Streck 218976 Cell-Free RNA BCTBlood Collection Tube基因检测无创采血管,Streck Cell-Free DNA Urine Preserve游离DNA尿液保存管规格:100管/盒,应用于:唐氏无创筛查采血管,游离DNA采血管,游离RNA采血管等等。华雅再生医学 现货供应:BD 362761 Vacutainer® CPT™ Cell Preparation Tube 肝素钠单核细胞制备管/采血管,60支/盒。

    Streck218976Cell-FreeRNA BCT游离RNA样本收集管

    概要和原则

    1.可以通过样品处理,运输和加工来影响cf-RNA的准确分析,从而导致裂解的有核血细胞和随后的细胞RNA释放。此外,由于...引起的cf-RNA降解核酸酶活性可能有问题。

    2.包含在Streck's Cell-Free RNA BCT中的防腐剂可防止有核血细胞释放细胞RNA,并抑制核酸酶介导的cf-RNA降解,有助于整体两个cfRNA1,2,3和细胞RNA的稳定

    3.Streck218976Cell-FreeRNA BCT增强了cf-RNA和细胞RNA的纯化,检测和分析。样本收集在Streck218976Cell-FreeRNA BCT在18°C至25°C之间的温度下稳定长达7天,方便样品收集,运输和储存。

    试剂

    Streck218976Cell-FreeRNA BCT含有抗凝血剂K3EDTA,核酸酶抑制剂,代谢抑制剂和细胞防腐剂在液体介质中。

    注意:Streck218976Cell-FreeRNA BCT中的试剂表现为红色是正常现象。

    注意事项

    1.仅供研究使用。不用于诊断程序。

    2.不要将细胞冻结在Streck218976Cell-FreeRNA BCT中,因为会导致破裂。

    3.到期后不要使用管子。

    4.不要使用管子收集要注入患者的物质。

    5.产品预计供应使用。不要将其他组分稀释或添加到Streck218976Cell-FreeRNA BCT中。

    6.过量填充或灌装不足的管会导致不正确的血液与添加剂的比例,并可能导致不正确分析结果或产品性能差。

    警告

    a.玻璃易碎,处理时应采取预防措施。

    b.所有与它们接触的所有生物标本和材料都被认为是生物危害,应该被视为能够传播感染,按照生物安全法规处理,避免与皮肤和粘膜接触。

    c.产品应与传染性医疗废物一起处置。

    d.通过轻轻地将塞子从一侧摇动到另一侧或通过抓住来取出并重新插入塞子同时扭转和拉动。用于止动器拆卸的“拇指滚动”程序不推荐,可能导致管破裂和损伤。

    存储和稳定性

    1.在18°C至30°C储存时,空白的Streck218976Cell-FreeRNA BCT在有效期内稳定。

    2.空载Streck218976Cell-FreeRNA BCT长达14天的时间,从2°C至40°C的短期储存是可以接受的。

    3.不要冻结未经充填的Streck218976Cell-FreeRNA BCT。极端运输可能需要适当的绝缘

    温度条件。

    4.在Streck218976Cell-FreeRNA BCT中收集的血液样品在储存之前可以稳定长达7天

    18°C至25°C。

    产品检测说明

    1.多浑浊或沉淀可见。

    2.如果发生产品恶化的迹象,请联系Streck技术服务部门

    使用说明

    1.根据CLSI GP41-A65静脉穿刺收集标本。

    防止回流 - 由于Streck218976Cell-FreeRNA BCT含有化学添加剂,重要的是避免可能从管回流。

    为防止回流,请遵守以下注意事项:

    a.在收集过程中,将患者的手臂置于向下的位置。

    b.将止动器的管子保持在最高位置,以使管内容物不接触止动器或样品收集期间针头的末端。

    c.一旦血液开始流入管子,或者在2分钟内才能释放止血带。

    2.遵循CL41 GP41-A65中概述的排列顺序的建议。可以绘制Streck218976Cell-FreeRNA BCT后EDTA管和氟化草酸盐(糖酵解抑制剂)管。如果是Streck218976Cell-FreeRNA BCT管立即按照抽取顺序的肝素管,Streck建议收集非添加剂或EDTA在Streck218976Cell-FreeRNA BCT中收集之前,将其作为废液管。

    3.完全填充管。

    4.从适配器上取下管子,并轻轻搅拌8〜10次。不足或延迟混合可能导致不正确的分析结果或差的产品性能。一次反转是一个完整的转折的手腕,180度和背面如下图所示:

    5.在推荐的温度范围内收集,运输和储存管子。

    注意:

    1.为获得最佳效果,建议使用21G或22G针头。当使用较小的规格针时,可能会观察到较慢填充时间。

    2.当使用用于静脉穿刺的翼状(蝴蝶)收集套装,Streck Cell-Free RNA BCT是第一个拉伸管,先应该部分拉出非添加剂或EDTA废弃管,以消除空气或“死空间”从管道。

    RNA提取

    无细胞血浆RNA和细胞RNA的提取可以使用包括蛋白酶K处理步骤的大多数市售试剂盒来完成。

    无细胞血浆RNA步骤1.为了分离血浆,在室温下以300×g离心全血20分钟。步骤2.取下上层等离子体层并转移到新的锥形管(未提供)。步骤3.以5000xg离心等离子体10分钟。步骤4.每个试剂盒制造商说明隔离无细胞RNA。为了获得最佳结果,在提取无细胞RNA时,在离液盐存在下,在60℃下,包括蛋白酶K处理步骤(≥30mAU/ mL消化)1小时。

    细胞RNA步骤

    1.为了分离白细胞,要么将红细胞裂解并洗涤,要么离心全血并收集血沉棕黄层。步骤

    2.分离每个试剂盒制造商说明书的基因组RNA。为了获得最佳结果,在提取细胞基因组RNA时,在离液盐存在下,在60℃下,包括蛋白酶K处理步骤(≥30mAU/ mL消化)2小时。

    注意:

    1.无细胞RNA BCT不稀释血液样品;因此,不需要稀释因子校正。

    2.与大多数临床实验室标本一样,溶血,黄疸和脂血症可能影响用无细胞RNA BCT保存的血液样品获得的结果。

    英文介绍

    Cell-Free RNA BCT ®

    INSTRUCTIONS FOR USE

    Cell-Free RNA BCT® is a direct-draw whole blood collection tube intended for collection, stabilization and transportation of cell-free plasma RNA. Cell-Free RNA BCT also stabilizes and preserves cellular RNA in white blood cells. This product has not been cleared by the U.S. Food and Drug Administration for In Vitro Diagnostic Use. The product is For Research Use Only. Not for use in diagnostic procedures.

    SUMMARY AND PRINCIPLES

    Accurate analysis of cf-RNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular RNA. Additionally, degradation of cf-RNA due to nuclease activity can be problematic.

    The preservative reagent contained in Streck’s Cell-Free RNA BCT stabilizes nucleated blood cells, preventing the release of cellular RNA, and inhibits nuclease mediated degradation of cf-RNA, contributing to the overall stabilization of both cfRNA1,2,3 and cellular RNA.4

    Cell-Free RNA BCT enhances purification, detection and analysis of cf-RNA and cellular RNA. Samples collected in Cell-Free RNA BCT are stable for up to 7 days at temperatures between 18 °C to 25 °C, allowing convenient sample collection, transport and storage.

    REAGENTS

    Cell-Free RNA BCT contains the anticoagulant K3EDTA, nuclease inhibitors, metabolic inhibitors and a cell preservative in a liquid medium.

    Note: It is normal for the reagent in Cell-Free RNA BCT to have a reddish appearance.

    PRECAUTIONS

    1. For Research Use Only. Not for use in diagnostic procedures.

    2. Do not freeze specimens in Cell-Free RNA BCT as breakage could result.

    3. Do not use tubes after expiration date.

    4. Do not use tubes for collection of materials to be injected into patients.

    5. Product is intended for use as supplied. Do not dilute or add other components to Cell-Free RNA BCT.

    6. Overfilling or underfilling of tubes will result in an incorrect blood-to-additive ratio and may lead to incorrect analytic results or poor product performance.

    CAUTION

    a. Glass has the potential for breakage; precautionary measures should be taken during handling.

    b. All biological specimens and materials coming in contact with them are considered biohazards and should be treated as if capable of transmitting infection. Dispose of in accordance with federal, state and local regulations. Avoid contact with skin and mucous membranes.

    c. Product should be disposed with infectious medical waste.

    d. Remove and reinsert stopper by either gently rocking the stopper from side to side or by grasping with a simultaneous twisting and pulling action. A “thumb roll”procedure for stopper removal is NOT recommended as tube breakage and injury may result.

    7.SDS can be obtained at www.streck.com or by calling 800-843-0912.

    STORAGE AND STABILIT

    1. When stored at 18 °C to 30 °C, empty Cell-Free RNA BCT is stable through expiration date.

    2. Short-term storage from 2 °C to 40 °C is acceptable for empty Cell-Free RNA BCT for up to 14 days.

    3. Do not freeze unfilled Cell-Free RNA BCT. Proper insulation may be required for shipment during extreme temperature conditions.

    4. Blood samples collected in Cell-Free RNA BCT are stable for up to 7 days when stored between 18 °C to 25 °C.

    INDICATIONS OF PRODUCT DETERIORATION

    1. Cloudiness or precipitate visible.

    2. If indications of product deterioration occur, contact Streck Technical Services at 800-843-0912 or technicalservices@streck.com.

    INSTRUCTIONS FOR USE

    For a video demonstration, visit www.streck.com/mixing.

    1. Collect specimen by venipuncture according to CLSI GP41-A65.

    Prevention of Backflow - Since Cell-Free RNA BCT contains chemical additives, it is important to avoid possible backflow from the tube.

    To guard against backflow, observe the following precautions:

    a. Keep patient’s arm in the downward position during the collection procedure.

    b. Hold the tube with the stopper in the uppermost position so that the tube contents do not touch the stopper or the end of the needle during sample collection.

    c. Release tourniquet once blood starts to flow in the tube, or within 2 minutes of application.

    2. Follow recommendations for order of draw outlined in CLSI GP41-A65. Cell-Free RNA BCT can be drawn after the EDTA tube and before the fluoride oxalate (glycolytic inhibitor) tube. If a Cell-Free RNA BCT tube immediately follows a heparin tube in the draw order, Streck recommends collecting a non-additive or EDTA tube as a waste tube prior to collection in the Cell-Free RNA BCT.

    3. Fill tube completely.

    4. Remove tube from adapter and immediately mix by gentle inversion 8 to 10 times. Inadequate or delayed mixing may result in incorrect analytical results or poor product performance. One inversion is a complete turn of the wrist, 180 degrees, and back per the figure below:

    5. After collection, transport and store tubes within the recommended temperature range.

    Note:

    1. For best results, a 21G or 22G needle is advised. Slower fill times may be observed when using a smaller gauge needle.

    2. When using a winged (butterfly) collection set for venipuncture and the Streck Cell-Free RNA BCT is the first tube drawn, a non-additive or EDTA discard tube should be partially drawn first in order to eliminate air or “dead space”from the tubing.

    RNA EXTRACTION

    Extraction of cell-free plasma RNA and cellular RNA can be accomplished using most commercially available kits that include a Proteinase K treatment step.

    Cell-free Plasma RNA Step 1. To separate plasma, centrifuge whole blood at 300 x g for 20 minutes at room temperature. Step 2. Remove the upper plasma layer and transfer to a new conical tube (not provided). Step 3. Centrifuge the plasma at 5000 x g for 10 minutes. Step 4. Isolate cell-free RNA per kit manufacturer instructions. For optimal results, include a Proteinase K treatment step (≥30 mAU/mL digest) at 60 °C in the presence of chaotropic salts for 1 hour when extracting cell-free RNA.

    Cellular RNA Step 1. To separate the white blood cells, either lyse the red blood cells and wash, or centrifuge whole blood and collect the buffy coat layer. Step 2. Isolate genomic RNA per kit manufacturer instructions. For optimal results, include a Proteinase K treatment step (≥ 30 mAU/mL digest) at 60 °C in the presence of chaotropic salts for 2 hours when extracting cellular genomic RNA.

    Note:

    1. Cell-Free RNA BCT does not dilute blood samples; therefore, no dilution factor correction is necessary.

    2. As in the case with most clinical laboratory specimens, hemolysis, icterus and lipemia may affect the results obtained on blood samples preserved with Cell-Free RNA BCT.

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