AdvanceBio Endo F2，重组（原 ProZyme）. 该酶裂解高甘露糖型和二天线型复合 N-糖的壳二糖 (GlcNAc-GlcNAc) 核心。包括 5X 反应缓冲液，稀释后得到 200 mmol/L 醋酸钠，pH 值为 4.5。
The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.
Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase.