Parameter | CNBr-Activated Sepharose 4 Fast Flow, 10 g |
---|---|
Chromatography technique | Affinity coupling |
BioProcess resin | Yes |
Ligand | Cyanogen bromide activated |
Particle size, d50V1 | ~90 μm |
Group To be Coupled | Primary amines |
Matrix | 4% cross-linked agarose |
Particle Size | 45 μm-165 μm |
pH stability, operational2 | 3–11 |
pH stability, CIP | 2–11 (ligand dependent) |
Pressure/Flow Specification | Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
Storage | Unsused: 2 to 8°C - Used: 2 to 8°C in 20% Ethanol |
Chemical stability | Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment |
Certificate of Analysis | Yes |
Pack size | 10 g |