Recombinant Human/Mouse/Rat Activin A Protein Summary
Product Specifications
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50for this effect is 0.2?1.2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 2.5 x 10
3units/mg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626). Specific activity is for reference purposes only and is not routinely tested.
Source
Chinese Hamster Ovary cell line, CHO-derived Activin A protein
Gly311-Ser426
Accession #
N-terminal Sequence
Analysis
Gly311
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
14 kDa, reducing conditions
24 kDa, non-reducing conditions