Coelenterazine-based luminescence detection kit for standard and HTS assays
QUANTI-Luc? Gold is an optimized kit for the detection of Lucia luciferaseand other coelenterazine-utilizing luciferases (e.g. Gaussia and Renilla luciferases).
The light signal produced by the luciferase reaction with the coelenterazine substrate is quantified using a luminometer and expressed as relative light units (RLU).
This two-component kit comprises:
QUANTI-Luc? Plus, a coelenterazine-containing reagent
QLC Stabilizer
This kit provides the user with two options for luciferase activity measurement:
QUANTI-Luc? Gold (QUANTI-Luc? Plus with QLC Stabilizer) allows enhanced light signal stability. This option is ideal for high-throughput screening (HTS), time-course studies, or when using a luminometer without injectors.
QUANTI-Luc? Plus alone (without QLC Stabilizer) allows enhanced light output detection (as compared to QUANTI-Luc?). This option is ideal for monitoring the cellular response to weak agonists.
Specifications
Flexible:Two options: enhanced light signal stability or enhanced light output
Cost effective:One pouch prepares 5 x 96-well plates or 2 x 1536-well plates
Practical:Reconstituted QUANTI-Luc? Gold is stable for 1 week at 4°C or 1 month at -20°C