Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H, R, Ca, M, Pm||WB, ChIP-seq, ChIP||Rb||Serum||Polyclonal Antibody|
11 zinc finger transcriptional repressor
11-zinc finger protein
CCCTC-binding factor (zinc finger protein)
transcriptional repressor CTCF
|Background Information||CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.|
HeLa nuclear extract.
Included Positive Antigen Control:
Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
|Presentation||Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.|