Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.
High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
Supplied as pre-swollen bulk (loose) resin.
Chemically stable with all commonly used aqueous buffers.
Suitable for batch GST tagged protein purification and scale-up.
Used in GST pull-down assays to study protein-protein interactions.
Parameter | Glutathione Sepharose 4B, 10 mL |
---|---|
Chromatography technique | GST-tagged protein purification |
Ligand | Glutathione |
Particle size, d50V1 | ~90 μm |
Matrix | Agarose, 4% |
Particle Size | 45 μm-165 μm |
Ligand Coupling Method | Epoxy activation |
Binding Capacity/ml Chromatography Medium2 | > 25 mg horse liver GST/mL resin |
pH stability, operational3 | 4–13 |
pH stability, CIP4 | 4–13 |
Storage | 4 to 30°C, 20% Ethanol |
Chemical stability | Commonly used aqueous buffers |
Licensing | For licensing information, see the Licensing Statements page in the About Us section. |
Pack size | 10 mL |