Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.
High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
Supplied as pre-swollen bulk (loose) resin.
Chemically stable with all commonly used aqueous buffers.
Suitable for batch GST tagged protein purification and scale-up.
Used in GST pull-down assays to study protein-protein interactions.
Parameter Glutathione Sepharose 4B, 10 mL Chromatography technique GST-tagged protein purification Ligand Glutathione Particle size, d50V1 ~90 μm Matrix Agarose, 4% Particle Size 45 μm-165 μm Ligand Coupling Method Epoxy activation Binding Capacity/ml Chromatography Medium2 > 25 mg horse liver GST/mL resin pH stability, operational3 4–13 pH stability, CIP4 4–13 Storage 4 to 30°C, 20% Ethanol Chemical stability Commonly used aqueous buffers Licensing For licensing information, see the Licensing Statements page in the About Us section. Pack size 10 mL