InvivogenQUANTI-Blue? Solution rep-qbs

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产品报价： 面议

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产品热度：加载中

品　　牌：Invivogen

产品型号：rep-qbs

制造厂商：Invivogen

适用范围：全阶段

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　　Features and advantages of QUANTI-Blue? Solution:

• A convenient, easy and rapid means to detect and quantify SEAP and AP activity

• Short hands-on-time: < 10 min

• Easy to prepare: just mix QB Reagent and QB Buffer with water

• Highly sensitive

• Wide dynamic range

• High saturation threshold

• Allows for high-throughput screening (HTS)

　　In the presence of AP or SEAP, the color of QUANTI-Blue? changes from pink to purple/blue. The purple/blue color intensity reflects the activity of AP. The levels of AP can be determined qualitatively with the naked eye or quantitatively using a spectrophotometer at 620-655 nm.

　　Figures

　　QUANTI-Blue? Standard procedureQUANTI-Blue? High Throughput Screening (HTS) procedure

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　　Specifications

　　Physicochemical characterization: pH and solubility

　　Functional characterization:cellular assays using alkaline phosphatase of SEAP-expressing reporter cells.

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　　Contents

　　QUANTI-Blue? Solution is available in three pack sizes:

• rep-qbs : 5 x 1 ml of QB reagent + 5 x 1 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 25 x 96-well plates or 20 x 1536-well plates

• rep-qbs2 :10 x 1 ml of QB reagent + 10 x 1 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 50 x 96-well plates or 40 x 1536-well plates

• rep-qbs3 :1 x 20 ml of QB reagent + 1 x 20 ml QB buffer, sufficient to prepare QUANTI?Blue? Solution for 100 x 96-well plates or 80 x 1536-well plates

　　Store QB reagent and QB buffer at -20°C.

　　Reconstituted QUANTI-Blue? Solution is stable for 2 weeks at 2-8 °C and for 2 months at -20°C. Keep reconstituted QUANTI-Blue? away from light.