Selection antibiotic: cell culture tested, sterile reagent
Blasticidin is an efficient selection antibiotic that acts on both eukaryotic and prokaryotic cells.
Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery.
Resistance to blasticidin is conferred by the blasticidin resistance gene from Bacillus cereus (bsr), which codes for blasticidin-S deaminase [1].
Resistance to blasticidin is also conferred by the blastcidin S acetyltransferase gene (bls) from Streptoverticillum sp [2], and the blasticidin S deaminase gene (BSD) from Apergillus terreus [3].
Typically, mammalian cells are sensitive to blasticidin concentrations of 1-10 μg/ml, and bacteria to 25-100 μg/ml.
Cell death induced by blasticidin occurs rapidly, allowing for selection of transfected cell lines carrying a blasticidin resistance gene within one week.
1. Izumi M. et al., 1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. Exp.Cell Res.197:229-33.
2. Perez-Gonalez J. et al., 1990. Cloning and characterization of the gene encoding a blasticidin S acetyltransferase from Streptoverticillum sp. Gene. 86:129-34.
3. Kimura M. et al., 1994. Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219:653-9.
Specifications
Product concentration: 10 mg/ml
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 95% (HPLC)
Endotoxin level: < 1 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in blasticidin-sensitive and blasticidin-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in blasticidin-resistant cells
Contents
Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer.
This product is available in four pack sizes:
ant-bl-05: 5 x 1 ml (50 mg)
ant-bl-1: 10 x 1 ml (100 mg)
ant-bl-5: 50 x 1 ml (500 mg)
ant-bl-5b: 1 x 50ml (500 mg)
Blasticidin hydrochloride is also supplied as a powder:
ant-bl-10p: 1 g (powder)
Blasticidin is shipped at room temperature.
Upon receipt it should be stored at 4°C or -20°C.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Citations
2018 Nat Commun. 9(1):1727.
Linear mitochondrial DNA is rapidly degraded by components of the replication machinery.
Peeva V. et al.
2018 Nat Immunol. 19(5):453-463.
Hammer Q. et al.
