CD4 MicroBeads, human
CD4 MicroBeads were developed for the positive selection or depletion of CD4
+
cells by direct magnetic labeling. The most commonly used cell sources are peripheral blood, leukapheresis
1
, and cord blood. In addition, T helper cells have also been isolated from lymph nodes, thymus, spleen, skin biopsies
2
, synovial fluids, and cell cultures.
Specifications for CD4 MicroBeads, human
Overview
CD4 MicroBeads were developed for the positive selection or depletion of CD4
+
cells by direct magnetic labeling. The most commonly used cell sources are peripheral blood, leukapheresis
1
, and cord blood. In addition, T helper cells have also been isolated from lymph nodes, thymus, spleen, skin biopsies
2
, synovial fluids, and cell cultures.
Detailed product information
Background information
CD4 is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimers on CD4
+
T helper cells. CD4 is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 molecule is the receptor for the human immunodeficiency virus. The CD4 antibody recognizes most thymocytes and about 65% of all peripheral blood T cells.
Downstream applications
Isolation or depletion of CD4
+
T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS? Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays
10
and cytotoxicity assays
3
, as well as for the generation of T cell lines
9
. CD4
+
T cells purified with CD4 MicroBeads have also been cultured and analyzed for
in vitro
cytokine production.
4
Furthermore, they have been used in co?culture experiments, e.g., for allogeneic stimulation by dendritic cells
1
or for B cell activation
2
.
T helper cells isolated by MACS
?
Tech-nology were also used for studies on viral infections, e.g., for
in vitro
infections of cells with HIV
5
, to determine the infectivity of HIV-infected follicular dendritic cells
6
, immune reconstitution after anti-viral therapy
7
, and for monitoring of immune abnormalities in children of HIV-infected mothers
8
.
Columns
For positive selection: MS, LS, XS, or autoMACS
?
Columns. For depletion: LD, D, or autoMACS Columns.
