概述
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep? Releasable RapidSpheres?. Then, bound magnetic particles are removed from the EasySep?-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep? Dextran RapidSpheres?. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD8+ T cells that are immediately ready for downstream applications. Effector memory CD8+ T cells can be isolated from the CD62L- fraction.
Advantages
? Easy-to-use and column-free
? Up to 93% purity
? No-wash removal of EasySep? Releasable RapidSpheres?
Components
EasySep? Human Central and Effector Memory CD8+ T Cell Isolation Kit (Catalog #17869)
EasySep? Human Memory CD8+ T Cell Isolation Cocktail, 0.5 mL
EasySep? Dextran RapidSpheres?, 1 mL
EasySep? Human CD62L Positive Selection Cocktail, 1 mL
EasySep? Releasable RapidSpheres?, 1 mL
EasyeSep? Release Buffer, 1 mL
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? Easy 50 EasySep? Magnet (Catalog #18002)
? EasyEights? EasySep? Magnet (Catalog #18103)
Subtype
细胞分选试剂盒
Cell Type
CD8+T细胞
Species
人
Sample Source
白细胞去除术, PBMC
Selection Method
正选
Application
细胞分离
Brand
EasySep
Area of Interest
免疫学